Lois was part of a military family as her father Sam Lane was actively enlisted in the United States military. She and her sister Lucy grew up on an army base, and Lois received martial arts training from her father.
Lois always liked to be on top; she was not above taking risks to get a story as well as using aggressive tactics to accomplish her goals. These have included using informants; this technique has gotten her into trouble in two separate instances. The first was where Lex Luthor discovered that one of his scientist provided Lois with information and used him to lure Superman into a trap. The second being when she recruited an engineer who worked for LexCorp to provide information. He promptly became obsessed with, and tried to kill her out of revenge. She is an award winning reporter who has traveled a great deal and can be easily classified as the newspaper's star reporter.
When she first met Clark Kent, she dismissed him as an amateur. Eventually, they became friends and would work together regularly. Although, Clark was able to beat Lois out of story on a few occasions. Examples include when Clark was able to clear a convicted killer waiting to die on death row and his breaking the story of Superman's research pact with S.T.A.R. Labs. She eventually learned to respect Clark and would come to trust his judgment and abilities, including taking it hard when he faked his own death. She has had a few significant romantic relationships as well. She previously had dated Luthor, until she eventually dumped him. She even had a brief relationship with Bruce Wayne before discovering his secret identity as Batman. She declined a further romantic relationship with him, saying that there were things about his life she didn't want to know, though later she explained she almost had second thoughts about their brief relationship.
Lois was at first skeptical of Superman but like so many others in Metropolis grew to love him as their hero and symbol. Lois was saved by Superman on multiple occasions, including when terrorists took over Air Force One and when the deranged criminal Toyman kidnapped her as part of his scheme against Bruno Manheim. At one point, during Superman's brainwashing by Darkseid, she enlisted her father's help in obtaining the necessary security clearance so she could break Superman out of a military installation. In the end, however, she eventually got closer to him and ended up dating him. After the Cadmus Crisis, it was never made clear whether or not Lois had figured out that Superman and Clark Kent were the same person.
Lois's determined journalistic skills were her primary skills. Being the daughter of an army officer, she also had a fair knowledge of self-defense and other skills.
Superman/Batman Annual #4, a story by Paul Levitz, elaborates on the world of Batman Beyond. Lois is revealed to have died roughly ten years prior, leaving Superman distraught and less connected with humanity; even going so far as to leave Earth after locking Luthor away in the Phantom Zone.